Education

Research Description

Microalgae culture and bioprocessing; Fermentations; Sustainable agriculture; Artificial photosynthesis and bioseparations; Biomass thermochemical conversion; Microbial and bio-fuel cells; Biodiesel quality control and utilization

Honors and Awards

• NCSU University Faculty Scholar, 2017
• ASABE Rain Bird Engineering Concept of the Year Award, 2016
• ASABE Superior Paper Award, 2015
• ASABE New Holland Young Researcher Award, 2012
• NSF CAREER Award, 2010
• Mid-central section of the ASABE Distinguished Service Award, 2010
• ASABE Superior Paper Award, 2006
• ASABE Boyd-Scott Graduate Research Award 1st place (Ph.D.), 2005
• AOCABFE Scholarly Achievement Award, 2005
• AOCABFE Research Paper Award, 2nd place, 2005

Publications

Algal biorefinery to value-added products by using combined processes based on thermochemical conversion: A review

Fan, L., Zhang, H., Li, J., Wang, Y., Leng, L., Li, J., … Zhou, W. (2020). [Review of , ]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, 47. https://doi.org/10.1016/j.algal.2020.101819

Algal cell lysis by bacteria: A review and comparison to conventional methods

Wang, M., Chen, S., Zhou, W., Yuan, W., & Wang, D. (2020). [Review of , ]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, 46. https://doi.org/10.1016/j.algal.2020.101794

Biochar production and applications in agro and forestry systems: A review

Wang, D., Jiang, P., Zhang, H., & Yuan, W. (2020). [Review of , ]. SCIENCE OF THE TOTAL ENVIRONMENT, 723. https://doi.org/10.1016/j.scitotenv.2020.137775

Composition and secondary structure of proteins isolated from six different quinoa varieties from China

Wang, X., Zhao, R., & Yuan, W. (2020), JOURNAL OF CEREAL SCIENCE, 95. https://doi.org/10.1016/j.jcs.2020.103036

Desirability function approach for optimization of enzymatic transesterification catalyzed by lipase immobilized on mesoporous magnetic nanoparticles

Khoobbakht, G., Kheiralipour, K., Yuan, W., Seifi, M. R., & Karimi, M. (2020, October), RENEWABLE ENERGY, Vol. 158, pp. 253–262. https://doi.org/10.1016/j.renene.2020.05.087

Dynamics of microbial community and changes of metabolites during production of type I sourdough steamed bread made by retarded sponge-dough method

Wang, X., Zhu, X., Bi, Y., Zhao, R., Nie, Y., & Yuan, W. (2020), FOOD CHEMISTRY, 330. https://doi.org/10.1016/j.foodchem.2020.127316

Kinetic modeling of butyric acid effects on butanol fermentation by Clostridium saccharoperbutylacetonicum

Zhou, Q., Liu, Y., & Yuan, W. (2020), NEW BIOTECHNOLOGY, 55, 118–126. https://doi.org/10.1016/j.nbt.2019.10.004

The Effect of Gasification Conditions on the Surface Properties of Biochar Produced in a Top-Lit Updraft Gasifier

James, A. M. R., Yuan, W., Wang, D., Wang, D., & Kumar, A. (2020), APPLIED SCIENCES-BASEL, 10(2). https://doi.org/10.3390/app10020688

Type I sourdough steamed bread made by retarded sponge-dough method

Wang, X., Zhao, R., & Yuan, W. (2020), FOOD CHEMISTRY, 311. https://doi.org/10.1016/j.foodchem.2019.126029

Catalysts evaluation for production of hydrogen gas and carbon nanotubes from the pyrolysis-catalysis of waste tyres

Li, W., Wei, M., Liu, Y., Ye, Y., Li, S., Yuan, W., … Wang, D. (2019), INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, 44(36), 19563–19572. https://doi.org/10.1016/j.ijhydene.2019.05.204

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Grants

Biochar-Based Soil Nutrient Carriers: Production, Processing and Performance

US Dept. of Agriculture (USDA) - National Institute of Food and Agriculture(7/15/19 - 7/14/22)

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Biochar-Based Soil Nutrient Carriers: Production, Processing and Performance

Sponsor: US Dept. of Agriculture (USDA) - National Institute of Food and Agriculture

Start Date: 7/15/19

End Date: 7/14/22

Abstract

It is extremely important and urgent to develop fertilizers that can not only enhance crop yields, but also protect the environment by reducing nutrient losses! This project will develop a biochar-based organic fertilizer from an engineered biochar-manure co-aging (EBMCA) process. The resultant EBMCA fertilizer is expected to be superior to sole manure or biochar in that it enhances soil N retention capability and improves the synchrony between nutrient supply and nutrient need due to biochar’s unique characteristics: 1) A high ion exchange capacity (IEC) and a modifiable nature of IEC via abiotic and biotic oxidation processes; 2) A large surface area and porosity for carrying abundant and diverse beneficial microbes; 3) A powerful adsorption for retaining, stabilizing and buffering N from manure and soil. This project aims to characterize a number of properties and processes during the production and application of the fertilizer, includes five objectives: 1) Correlate gasification conditions with the chemical and physical properties of reproducible biochars as a bioenergy co-product in low-temperature gasification; 2) Elucidate the effects of the biochar quality and quantity on nutrient retention and/or loss during manure co-aging process; 3) Examine the impacts of the biochar-based organic fertilizer on soil properties and processes underlying soil nutrient release patterns and greenhouse gas emissions; 4) Evaluate the performance of the biochar-based organic fertilizer in terms of crop nutrient use efficiency, performance/quality, and yield;

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Renewable Paving Binders from Top-lit Updraft Kilning of Biomass

National Science Foundation (NSF)(6/15/15 - 5/31/21)

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Renewable Paving Binders from Top-lit Updraft Kilning of Biomass

Sponsor: National Science Foundation (NSF)

Start Date: 6/15/15

End Date: 5/31/21

Abstract

The long-term goal of the proposed research is develop a sustainable alternative to asphalt binder in pavements. For an alternative binder to be viable as a replacement to asphalt cement, it must (a) be derived from renewable sources that can be produced economically in mass quantities, (b) require less energy and produce fewer emissions than the production and construction of concrete using current asphalt binder technology, and (c) demonstrate equal or superior performance over asphalt binder. To best meet these objectives, bio-binders will be produced using thermo-chemical conversion of biomass. Biomass feedstock and thermo-chemical conversion operating parameters will be varied in order to link inputs to produce a viable paving binder. Rigorous analyses of bio-binder chemical composition and properties will be conducted to assess the viability of bio-binders produced for use in pavements. In addition, preliminary trials of producing bio-binder – aggregate mixtures will be conducted using conventional laboratory methods for producing asphalt mixtures. Performance testing of bio-binder – aggregate mixtures will be conducted to assess their performance and to link critical bio-binder properties to the resultant performance of mixtures. In addition, life cycle analysis will be conducted to assess the sustainability of bio-binders compared to petroleum-based asphalts.

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Collaborative Research: Artificial Photosynthesis Based on Archaeal Lipids and Proteins for Biofuels

National Science Foundation (NSF)(9/01/14 - 8/31/18)

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Collaborative Research: Artificial Photosynthesis Based on Archaeal Lipids and Proteins for Biofuels

Sponsor: National Science Foundation (NSF)

Start Date: 9/01/14

End Date: 8/31/18

Abstract

The main objective of this research is to fabricate a highly efficient and durable, membrane-based artificial photosynthesis device using novel lipids and enzymes from thermoacidophiles. The system will be capable of converting sunlight, carbon dioxide, and water into carbohydrates for the production of biofuels such as ethanol. Microbe growth, protein and lipid purification and characterization, membrane biophysics, biochemical assays, various spectroscopic techniques such as fluorescence, circular dichroism, UV-visible and infrared, as well as Micro-Electro-Mechanical-System (MEMS) technology will be employed.

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Bio-Binders From Hydrothermal Conversion of Biomass for Paving Applications

NCSU Research and Innovation Seed Funding Program(1/01/13 - 12/31/13)

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Bio-Binders From Hydrothermal Conversion of Biomass for Paving Applications

Sponsor: NCSU Research and Innovation Seed Funding Program

Start Date: 1/01/13

End Date: 12/31/13

Abstract

Currently, over 90% of pavements worldwide are constructed with an asphaltic surface, which results in use of 30 million tons of asphalt annually in the US alone. Asphalt is a byproduct of refining petroleum, a non-renewable resource and thus, supplies are diminishing. There is a need for development of alternative binders from bio-renewable resources. This project will investigate the use of bio-binders produced via hydrothermal conversion of biomass as an alternative to asphalt. Both micro algae and corn cob will be evaluated as biomass feedstock for production of bio-binders. Experimental characterization of bio-binders will be conducted to assess their potential use as paving binders.

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GOALI/Collaborative Research: Additive Manufacturing for a Highly Efficient Artificial Photosynthesis Device with Multi-layer Interconnected Channels and Micro-Porous Structures

National Science Foundation (NSF)(6/15/13 - 5/31/18)

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GOALI/Collaborative Research: Additive Manufacturing for a Highly Efficient Artificial Photosynthesis Device with Multi-layer Interconnected Channels and Micro-Porous Structures

Sponsor: National Science Foundation (NSF)

Start Date: 6/15/13

End Date: 5/31/18

Abstract

The main objective of this research is to fabricate an artificial photosynthesis device that is capable of converting sunlight, CO2 and water into sugars/glucose for the production of biofuels. Additive manufacturing (AM) enhanced by high-resolution heterogeneous material printing technology and multi-function nozzle array will be investigated to design and build the innovative device with multi-layer interconnected channels and micro-porous structures. This research will enable manufacturing and deployment of large-scale solar conversion systems that not only mimic the nature process of photosynthesis for the production of biofuels, but also make these reactions independent of the life of nature plants.

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Bio-Hydrogen Generation in a Sequential Fermentation-Microbial Electrolysis System

NCSU Faculty Research & Professional Development Fund(7/01/12 - 6/30/13)

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Bio-Hydrogen Generation in a Sequential Fermentation-Microbial Electrolysis System

Sponsor: NCSU Faculty Research & Professional Development Fund

Start Date: 7/01/12

End Date: 6/30/13

Abstract

The long term goal of this new program is to enable cost-effective bio-hydrogen production using waste/low-value materials, such as hemicellulose in biomass. The objective of this proposal is to understand the effect of pH on bio-H2 generation of a sequential fermentation-microbial electrolysis system (SFMES) using xylose as the carbon source. Clostridium tyrobutyricum will be used to decompose xylose to make organic acids and H2 in the first step. The organic acids will then be fed to a microbial electrolysis cell (MEC) in the second step to generate H2 on the cathode side. With the input of a low-cost, widely available carbon source, hydrogen will be produced in both steps. The project will lay the foundation for the PI (a new tenure-track faculty) to establish a new research program focusing on bio-H2 production, and will generate preliminary data for future grant proposals.

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Nozzle design and fluid dynamics for algal cell disruption and lipid extraction

NCSU Research and Innovation Seed Funding Program(7/01/12 - 6/30/13)

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Nozzle design and fluid dynamics for algal cell disruption and lipid extraction

Sponsor: NCSU Research and Innovation Seed Funding Program

Start Date: 7/01/12

End Date: 6/30/13

Abstract

The goal of this proposal is to foster collaboration between two junior faculty in Biological Engineering (Dr. Wayne Yuan, CALS) and Mechanical Engineering (Dr. Tiegang Fang, COE) to generate preliminary data for upcoming NSF proposals. The proposed work will focus on a grand challenge in algal biofuel production ? algal cell disruption and lipid extraction. The effect of spray nozzle design and processing conditions on algae cell disruption and lipid extraction, as well as the mechanism (fluid dynamics) of the proposed nozzle spraying system will be studied. This project integrates research in algal cell mechanics, cell-based multiparametric detection, advanced fluid dynamics, and mechanical system design. It will lead to submission of grant proposals to the CMMI and CBET divisions of NSF, and will eventually contribute to economical algal biofuel manufacturing that will greatly benefit the U.S. economy and energy security, as well as society and the environment in general.

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EAGER/Collaborative Research: Solid Freeform Fabrication of a Conceptual Artificial Photosynthesis Device

National Science Foundation (NSF)(1/01/12 - 7/31/13)

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EAGER/Collaborative Research: Solid Freeform Fabrication of a Conceptual Artificial Photosynthesis Device

Sponsor: National Science Foundation (NSF)

Start Date: 1/01/12

End Date: 7/31/13

Abstract

The main objective of this research is to fabricate an artificial photosynthesis device that is capable of converting sunlight, CO2 and water into sugars for the production of biofuels. Solid freeform fabrication (SFF) enhanced by high-resolution heterogeneous printing technology will be investigated to design and build the innovative device with multi-layer interconnected channels and micro-porous structures. This research will enable manufacturing and deployment of large-scale solar conversion systems that not only mimic the nature process of photosynthesis for the production of biofuels, but also make these reactions independent of the life of nature plants.

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CAREER: Multi-Scale Structured Solid Carriers Enabling Algae Biofuel Manufacturing in the Ocean

National Science Foundation (NSF)(6/01/12 - 6/30/18)

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CAREER: Multi-Scale Structured Solid Carriers Enabling Algae Biofuel Manufacturing in the Ocean

Sponsor: National Science Foundation (NSF)

Start Date: 6/01/12

End Date: 6/30/18

Abstract

The long-term career goal of the PI is to enable economically viable energy manufacturing from renewable sources such as algae. The PI's vision is to produce algae, for biofuel manufacturing, on solid carriers (e.g., thin sheets of metals or polymers) that float in the ocean. The research objective of this CAREER proposal is to test the hypothesis that micro- and meso-scale structures of solid carriers enhance attachment of algae. Such structures (e.g., dimples or channels) will be fabricated on the surface of the carrier. Knowledge obtained from this research will foster design and manufacturing of solid carriers ? the major equipment proposed for manufacturing algae biofuels in the ocean. The approach includes (1) experimental study of cell attachment to structured carriers, (2) physics-based modeling of cell-carrier interactions, and (3) prediction of the effect of carrier structures on algae attachment using the surface element integration (SEI) technique. The education objective of this proposal is to impart a system-level integration of a manufacturing-education-enhancement based theme at high school, undergraduate, and graduate levels by (1) exposure of undergraduate and graduate students to interdisciplinary research through NSF-funded IGERT and REU programs and the Biomass Technologies Certificate Program at Kansas State University (KSU), (2) K-12 outreach through existing outreach programs at KSU, (3) course and curriculum development in energy manufacturing, and (4) involvement of women and minorities through KSU mentoring programs.

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Value-Added Utilization of Biochar in Syngas Cleanup and Conditioning

US Dept. of Agriculture (USDA)(1/01/12 - 10/15/14)

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Value-Added Utilization of Biochar in Syngas Cleanup and Conditioning

Sponsor: US Dept. of Agriculture (USDA)

Start Date: 1/01/12

End Date: 10/15/14

Abstract

The goal of this project is to improve the economic and environmental sustainability of biomass gasification through value-added utilization of biochar byproduct and effective syngas cleanup and enhancement. The novelty of the proposed work lies in two aspects: (1) it develops an inexpensive catalyst for effective syngas cleanup and conditioning and (2) it provides a value-added use of gasification by-product.

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